RTCB - tRNA-splicing ligase RtcB homolog - human protein (Function)
 
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Protein
Gene
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RTCB »  tRNA-splicing ligase RtcB homolog   [ EC 6.5.1.3 ]
 
Gene name:  RTCB
Family name: RtcB
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.  
3
  • UniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
1
  • UniProtKB KW
Metal ion bindingdefinition[GO:0046872]  
1
  • UniProtKB KW
Poly(A) RNA bindingdefinition[GO:0044822]  
2
  • UniProtKB
RNA ligase (ATP) activitydefinition[GO:0003972]  
2
  • UniProtKB
Vinculin bindingdefinition[GO:0017166]  
1
  • UniProtKB
GO biological process 
In utero embryonic developmentdefinition[GO:0001701] silver  
1
  • Ortholog Compara
Placenta developmentdefinition[GO:0001890] silver  
1
  • Ortholog Compara
tRNA splicing, via endonucleolytic cleavage and ligationdefinition[GO:0006388]  
2
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 6.5.1.3: ATP + (ribonucleotide)(n) + (ribonucleotide)(m) AMP + diphosphate + (ribonucleotide)(n+m).  
3
  • UniProtKB
  • HAMAP-Rule
It requires the following cofactor
 Mn(2+) : Binds 2 manganese ions per subunit.  
1
  • UniProtKB
Note 
Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP (PubMed24870230).  
2
  • UniProtKB
 

Keywords

Biological process 
tRNA processing  definition   [KW-0819]
Molecular function 
Ligase  definition   [KW-0436]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 51493
PRO: PR:Q9Y3I0