ATG4B - Cysteine protease ATG4B - human protein (Function)
 
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ATG4B »  Cysteine protease ATG4B   [ EC 3.4.22.- ]
 
Protein also known as:  Autophagy-related cysteine endopeptidase 1.
Gene name:  ATG4B
Family name: Peptidase C54
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.  
6
  • CuratedUniProtKB
GO molecular function 
Cysteine-type peptidase activitydefinition[GO:0008234]  
1
  • IDAUniProtKB
Endopeptidase activitydefinition[GO:0004175]  
1
  • IDABHF-UCL
Protein bindingdefinition[GO:0005515]  
7
  • IPIIntAct
  • IPIUniProtKB
GO biological process 
Autophagic vacuole assemblydefinition[GO:0000045]  
1
  • IGIUniProtKB
Autophagydefinition[GO:0006914]  
1
  • IGIUniProtKB
Positive regulation of autophagydefinition[GO:0010508]  
1
  • IDABHF-UCL
Positive regulation of protein catabolic processdefinition[GO:0045732] silver  
  • IEAOrtholog Compara
Protein transportdefinition[GO:0015031]  
  • IEAUniProtKB KW
Proteolysisdefinition[GO:0006508]  
2
  • IDABHF-UCL
  • IDAUniProtKB
Enzymatic activity 
It is regulated in the following manner
Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H(2)O(2) inhibits its activity.  
1
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Regulation of autophagy  hsa04140+23192  
 

Biophysicochemical properties

Kinetic parameters
KM 5.8 uM for GABARAP
KM 4.4 uM for GABARAPL1
KM 5.1 uM for MAP1LC3B
KM 6.1 uM for GABARAPL2

Keywords

Biological process 
Autophagy  definition   [KW-0072]
Protein transport  definition   [KW-0653]
Transport  definition   [KW-0813]
Ubl conjugation pathway  definition   [KW-0833]
Molecular function 
Hydrolase  definition   [KW-0378]
Protease  definition   [KW-0645]
Thiol protease  definition   [KW-0788]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: ATG4B
GenomeRNAi: 23192
PRO: PR:Q9Y4P1